Part:BBa_K1088007:Design

E. coli dxs-GFP fusion (lac promoter without lac inhibitor: IPTG uninducible)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2756
  Illegal SapI.rc site found at 946
  

Design Notes

We used USER cloning to assemble this part and therefore there are no scar sites. However we did insert a scarsite between the RBS and the start codon aswell as between the promoter and RBS, for optimal expression of our protein.

Source

The subparts of this composite part is derived from E. coli K-12 MG1655. The GFP derives from Aequorea victoria.